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Together, these results demonstrate that reprogramming BoNT/X protease to cleave gasdermin D is achievable through iterative substrate design, negative selection and circuit optimization
Experimental design Sixty (60) adult male Wistar rats (120-150 g) were maintained in the Animal House under controlled environmental conditions (25 2 C) at the National Research Centre in Giza, Egypt
Understanding concentration and dose calculations This section separates researchers who get consistent results from those who do not
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