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Protein concentration of the supernatant was then measured by BCA assay, followed by reduction in 10 mM DTT and alkylation in 20 mM IAM
Subsequently, this effect is reversed by the decreasing Nrf2 levels in the nucleus (nNrf2), leading to a decreased Keap1 level that is accompanied by a recovery of unbound Nrf2 because of a decreased sequestration in complexes with Keap1 (Fig 3C
3.7 Limitations and dual roles of the HSF/HSP system Although the HSP system constitutes a major defense mechanism by which the testes respond to heat stress, it is not infallible
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