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Sulfation (30-35%) - SAFE Safe elimination pathway DECREASES by 33% during pregnancy Limited capacityeasily saturated with higher or repeated doses Predominant pathway in fetuses/children but quickly overwhelmed 3
Excess GSH was removed by gel filtration with a 16/60 Superdex75 column (GE Healthcare) equilibrated in 20 mM Hepes (pH 7.5), 150 mM NaCl, and 1 mM EDTA
Structural plasticity among glutathione transferase phi members: natural combination of catalytic residues confers dual biochemical activities
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