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It was not until a genetically engineered strain of Pseudomonas denitrificans was used, in which eight of the genes involved in the biosynthesis of the vitamin had been overexpressed, that the complete sequence of methylation and other steps could be determined, thus fully establishing all the intermediates in the pathway
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Objective measurements (EEG, cardiovascular, and respiratory parameters) and subjective ratings (visual analog scales, awakening times) showed, assessed by Mann-Whitney U test, a normalization of severely disturbed sleep to normal sleep patterns
