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Lipid peroxidation was quantified by measuring the absorbance at 532 nm of the pink-colored complex formed by the reaction between thiobarbituric acid and MDA using a microplate reader (Infinite E Plex, Tecan, Mnnedorf, Switzerland), as previously described ( 2.11 Reduced GSH Reduced GSH levels were measured using a Reduced GSH Content Assay Kit (BC1175, Solarbio, Beijing, China) following the manufacturers instructions
Receptor desensitization: Continuous use beyond 68 weeks may lead to diminished response
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