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Some observational studies have raised concern with high selenium status
Diabet Med 38:e14655
"Melatonin, an ubiquitous metabolic regulator: functions, mechanisms and effects on circadian disruption and degenerative diseases"
For these assays, SUP-B15 cells were cultured in Iscoves modified Dulbeccos medium supplemented with 4 mM L-glutamine, 1.5 g l 1 sodium bicarbonate, 0.05 mM 2-mercaptoethanol, 80% RPMI-1640 and 5% FBS instead of 20% FBS to improve toxin activity
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