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Autofluorescence excited at 405 nm was collected from 430 to 480 nm and values were used to subtract autofluorescence bleeding into the roGFP channel as described previously (Schwarzlnder et al., Ratiometric image analysis Images were imported into a custom written MatLab (The MathWorks, www.mathworks.de) analysis suite (M.D

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Ganglion cell layer (GCL) volume was measured without pupil dilatation in both eyes of each patient by means of a 20 20 macular volume scan (centered on the macula with 512 A-scans and 25 B-scans aligned vertically with 16 averaged frames)

BMC Pregnancy and Childbirth, 24, 720