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Blotted membranes were incubated with 5% bovine serum albumin in PBS containing 0.05% Tween 20 for 60 min at room temperature and then incubated with primary antibodies against GSTP1 (Medical and Biological Laboratories, Nagoya, Japan), GSTA1 (Abcam, Cambridge, UK), GSTK1 (Abcam), GSTM1 (Abcam), GSTO1 (Abcam), GSTT1 (Abcam), GSTZ1 (Abcam), JNK (Cell Signaling Technology, Danvers, MA), phosphorylated JNK (R&D Systems, Minneapolis, MN), and GAPDH (Cell Signaling Technology)
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