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Metabolomics measures the chemical products of these genes and the enzymes they produce

BPC-157 Peptide Synthesized and Lyophilized in Canada by Koi Peptides Domestic Synthesis at a Canadian Facility The 15-residue chain comes together at Koi's Canadian production partner through standard Fmoc solid-phase chemistry, with the unusually high proline content (four prolines, including the Pro-Pro-Pro stretch at positions 3 to 5) handled through coupling protocols tuned for sterically hindered residues

Low capture genes with less than ten total counts summed across all samples were removed

An AriaMx Real-Time PCR system was used to perform real-time polymerase chain reaction (PCR) (Agilent Technologies Applied Biosystems, Carlsbad, CA, USA)