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Next, we monitored autophagic flux in GSH-depleted cells by using a tandem fluorescent-tagged LC3 lentivirus mRFP-GFP-LC3 expression system
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its function depends on the ATP-driven chaperone cycle and requires cooperation with HSP40 co-chaperones and nucleotide exchange factors (NEFs), which regulate substrate binding and release, promote correct protein folding, and prevent protein aggregation (Jiang et al., 2019)
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