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Therefore, future studies are warranted to ascertain whether Insl5 meets the necessary criteria to be considered a regulator of feeding given that the exogenous studies mentioned above utilize high doses of Insl5, although the initial work is promising

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The constructs CBS 516525 and CBS CD (residues 1413), along with single point mutations of CBS, were introduced using In-Fusion (Takara) or QuikChange (Agilent) mutagenesis and confirmed by sequencing