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When a research timeline extends past the working window, common practice is splitting the reconstituted solution into single-use volumes immediately after reconstitution freezing those aliquots at 20 C to limit cumulative damage

doi:10.1159/000342534

doi:10.1016/j.jdin.2025.01.012

Studies in obese rats have reported increased iron and hepcidin levels, as well as defects in mitochondria and endoplasmic reticula in the testis, which have been shown to be involved in ferroptosis [109]

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