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*Correspondence: Francesco Giorgino, [email protected] This article was submitted to Clinical Diabetes, a section of the journal Frontiers in Endocrinology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

The T7 promotor in the control plasmid should not lead to protein expression in mammalian cells

There are estrogen receptors pretty much everywhere in the body from the brain and eyes to ligaments and tendons so when those tissues are supported with hormone therapy, women can experience less brain fog and hot flashes, and fewer aches and pains (less frozen shoulder!)

also called fumarase) gene