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Anesthesiologists determine appropriate techniques based on individual risk assessment
Isolation of nuclear and cytoplasmic proteins Nuclear and cytoplasmic proteins were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit following the detailed instructions provided by the manufacturer (Thermo scientific, Rockford, IL, USA)
This result indicated that the cell membrane segment of the Wnt/-catenin pathway regulates liver zonation
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