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Kalaany and Sabatini had shown in NOD-SCID (non-obese diabetic, severe combined immunodeficient) mice that xenografted tumors with constitutive activation of AKT by either gains in PIK3CA or loss of PTEN are resistant against CR, and insensitive to insulin and IGF-1 treatment in vitro

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Molecular mechanisms of iron mediated programmed cell death and its roles in eye diseases

Tissue sections were rehydrated in 1xPBS, blocked for non-specific binding in 5% BSA and 0.1% triton for 1-hour at 4 C, and then stained with primary conjugated antibodies for cell and nuclear shape analysis