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To obtain a GLP1-RG s complex with good homogeneity and stability, we used the NanoBiT tethering strategy, in which the C-terminus of rat G1 was linked to HiBiT subunit with a 15-amino acid polypeptide (GSSGGGGSGGGGSSG) linker and the C-terminus of GLP-1R was directly attached to LgBiT subunit followed by a TEV protease cleavage site and a double MBP tag (Supplementary Fig
doi: 169
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