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The Trx1 loaded beads and lysates were incubated for 3 h on a rotator at 4C, then the reaction was stopped by adding NEM to a final concentration of 20 mM and incubating on ice for 5 min
Eur J Biochem 47:469474 Mohammad MK, Avila D, Zhang J et al (2012) Acrolein cytotoxicity in hepatocytes involves endoplasmic reticulum stress, mitochondrial dysfunction and oxidative stress
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