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Around 5 10 6 cells per 100-mm-diameter dish were transfected with the ecotropic packaging plasmid pCL-Eco (4 g) together with one of the following retroviral constructs (4 g): pMXs-Klf4, pMXs-Sox2, pMXs-Oct4 or pMXs-cMyc (obtained from Addgene) using Fugene-6 transfection reagent (Roche) according to the manufacturers protocol
Each batch of vitamin B12 1500 mcg injection is tested for quality, clarity, and sterility and is accompanied by a certificate of analysis
Methylcobalamin is the active, methylated form
These two delaying factors are why patients with low B12 levels are often prescribed B12 shots instead of oral supplements
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