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Human GLP-1R, human DNGs, and His 6 -tagged human G 1 and G 2 were expressed in sf 9 insect cells (Expression systems) using baculovirus and then infected with three separate baculoviruses at a ratio of 5:2:2 for GLP-1R, DNGs and G 1 2
1 H NMR (400 MHz, CDCl 3 ) 8.94 (t, J = 5.3 Hz, 2H, NH 2), 7.887.62 (m, 30H, ArH 30), 3.843.67 (m, 8H, H 2 -1 2 and H 2 -6 2), 3.07 (dd, J = 11.7, 5.8 Hz, 4H, H 2 -2 2), 1.871.76 (m, 4H, H 2 -4 2), 1.711.49 (m, 8H, H 2 -3 2 and H 2 -5 2)
Additionally, phentermine carries inherent risks for dependence and tolerance, particularly with long-term use
Ethics declarations Competing interests M.C., A.C., F.B., S.P
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