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After removing the spent medium, cells were incubated in 500 l complete MEM containing antibiotics (10 g ml 1 Rif, 50 g ml 1 Mox or 20 g ml 1 Van) with or without 0100 M KL1 or its analogues (KL27), 20 M BHA (Sigma) or 010 M BIX-01294, in the presence of 50100 g ml 1 Gen at 37 C for 624 h

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Using SH-SY5Y cells exposed to high glucose concentrations to induce hyperglycemia, disruptions in glucose sensing leads to activation of the phosphorylated protein kinase B (pAkt)/GSK-3 signaling pathway, and increased expression of BACE and A42, an effect that can be reversed via pretreatment with melatonin [62]