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Cells were incubated with the agonist for 15 min (except in the case of KRAS assays, where the incubation time was 60 min), and bioluminescence resonance energy transfer (BRET) measurements were taken using a Victor X4 plate reader (PerkinElmer) immediately after the addition of coelenterazine h (final concentration: 1 M)

Kamei, N

KLOW combines four structurally and mechanistically distinct peptides

Levocarnitine was not bound to plasma protein or albumin when tested at any concentration or with any species including the human.9 In a 9-week study, 12 ESRD patients undergoing hemodialysis for at least 6 months received levocarnitine injection 20 mg/kg three times per week after dialysis