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The protein was eluted in wash buffer with 250 mM imidazole and treated with Lamda Phosphatase (New England BioLab) and Alkaline Phosphatase (New England BioLab) overnight at 4 C
Microcomputed tomography (CT) and histomorphometric analysis Femura and tibiae obtained from mice were dissected free of soft tissue, fixed overnight in 10% neutral buffered formalin for 24 h, dehydrated in 70% ethanol for 3 days, and analyzed by a high resolution CT (SkyScan 1076 in-vivo CT, SKYSCAN) installed with softwares NRecon v1.6, CTan v1.9, and CTVol v2.0
Research use only FOXO4-DRI research peptide FOXO4-DRI is a synthetic D-retro-inverso peptide derived from a FOXO4 interaction region
A B12 deficiency can lead to a weakened immune system
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