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The formation of ice crystals can shear the delicate amino acid chains, and the stress of a freeze-thaw cycle often leads to irreversible degradation, rendering the peptide inactive
222 The major function of the endoplasmic reticulum (ER) is the synthesis and folding of secreted and transmembrane proteins, increasing evidence suggests that persistent ER stress is associated with the onset and progress of chronic metabolic disorders like obesity and IR
Journal of Biomaterials Science, Polymer Edition , 19(8), 969-988
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