A second PCR reaction was performed to attach dual indices and Illumina sequencing adapters using primers from the Nextera XT Index Kit (Illumina, San Diego, CA, USA)
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Notably, supplementation of DKO cells with L-carnitine alone was also able to increase both histone acetylation and cell proliferation in lipid depleted conditions (Figure 6B, C), suggesting that it may promote acetylcarnitine shuttling out of mitochondria
Wang S, Chen Y, Liang H, Chen Y, Shi M, Wu J, et al