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Briefly, whole Drosophila were fixed in 4% PFA for 12 h at 4 C, then rinsed 3 PBS for 3 h at RT with gentle shaking, and then immersed in FlyClear Solution-1 (8% THEED (2,2,2,2- (Ethylenedinitrilo)-tetraethanol) (Sigma-Aldrich), 5% Triton X-100 (Sigma-Aldrich) and 25% Urea (Fisher Scientific, in PBS) for 14 days at 37 C, as described previously 26

The exact number depends on your starting skin tone, desired results, dosage used, and individual response to treatment

But slowly, steadily, they're shrinking

If you want a protein boost from your liquid base, use skim milk (8 grams of protein per cup), soy milk (7 grams per cup), or ultra-filtered milk like Fairlife (13 grams per cup)