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The compounds peptide structure requires specific environmental conditions to prevent degradation and ensure experimental reliability
In this work, we firstly clarified that oridonin is a Michael acceptor molecule, reacting to thiol group of GSH by an electrophilic addition and covalent bonding
However, these two natural GLP-1R agonists are rapidly digested by the enzyme DPP-4 and cleared by the kidneys within 1-2 minutes, making them unsuitable for direct therapeutic use
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