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Factors such as tissue source, donor age, in vitro passage number, culture conditions (including hypoxic versus normoxic preconditioning), inflammatory priming, cryopreservation methods, and the lack of standardized potency assays can markedly influence both the intrinsic metabolic profile of MSCs and the composition of their therapeutic secretome, resulting in variable therapeutic efficacy [19,20,21,22,23,24]
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