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Description

The culture medium was replaced with neuronal conditioned serum-free B-27/Neurobasal medium (Gibco, Life Technologies, cat.# 17504-044 for B27 and cat.# 21103-049 for Neurobasal) 2 h after plating

NOTE - this can take up to 48hrs to be approved

GLP-1 type medications mimic a natural hormone called GLP-1 (glucagon-like peptide-1) which regulates appetite and food intake

Starting at the distal end of the tail (allows for re-injection further up the tail should the first attempt fail), place the needle at a 1520 angle ready for injection

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