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Total RNA was extracted from the cells of each treatment group by the TRIzol method, and the cDNA was synthesized by reverse transcription using total RNA as a template with Reverse Transcription Kit (Takara Bio Inc, Japan) at 37C for 15 min, 85 C for 5 s, and 4 C for 10 min
The experiment should be recorded for 5 min
Vitamin B12, also known as cobalamin, is a water-soluble vitamin that plays a vital role in various bodily functions
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