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View at Publisher | View at Google Scholar Hogan AE et al

Based on our experience with previously generated ecto-pHluorin-tagged membrane proteins 42,43,44 , we predicted that (1) ecto-pHluorin 1 integrins would emit little fluorescence in secretory vesicles due to the quenching of ecliptic pHluorin at acidic pH, and that (2) during exocytosis, ecto-pHluorin 1 integrin-loaded secretory vesicles fusing with the plasma membrane would expose their cargo to the neutral extracellular environment (pH~7.4) resulting in a burst of green fluorescence

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