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Transfection and reagents Cells were transfected using Lipofectamine 3000 (100022052, Invitrogen), FuGENE HD (E2311, Promega), Lipofectamine RNAiMAX (13778-150, Invitrogen) or PEI MAX (24765-100, Polysciences) according to the manufacturers instructions
Also referenced as DSIP , it is frequently used in laboratory models examining regulatory peptide signaling and cellular response mechanisms
Other immune cells, such as neutrophils, B cells, and T cells, are recruited by chemokines
Delivery times can vary, which may cause interruptions in medication schedules
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