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The sample was centrifuged at 160,000 g for 35 min and the supernatant was incubated with 1 mL TALON resin (Clontech) and 20 mM imidazole overnight at 4 C

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Glucagon Receptor Engagement : The addition of glucagon receptor activation is the core feature that separates Retatrutide from dual-agonist compounds

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