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And they also proved IFN downregulated the mRNA expression levels of both SystemX c subunits, leading to decreased GSH levels and inducing ferroptosis in tumor cells
Protein was eluted with 50 mM sodium phosphate pH 8.0, 100 mM imidazole, 150 mM NaCl buffer, dialyzed twice for three hours against 50 mM Tris-HCl pH 8.0, 1 mM EDTA buffer (1:400 volume protein:buffer) at 4 C and supplemented with 50% glycerol for storage at -20 C
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The SOD1 protein is found predominantly in the cytosol but also in the mitochondrial intermembrane space
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