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Lipids and oocyte developmental competence: the role of fatty acids and beta-oxidation
A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)
Tafuri A, Haimi J, Redner A, Andreeff M
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