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For lipid peroxidation level, Cells were treated with the fluorescent dye BODIPY 581/591 C11 (D3861, Thermofisher Scientific), which inserts into lipid membranes and allows for quantitative assessment of oxidized versus non-oxidized lipids by fluorescing green or red, respectively

An enzyme called g lutathione peroxidase then sparks the conversion of reduced glutathione to oxidized glutathione, a free radical itself

This provides evidence that OS might be the starting point, leading to further downstream processes like A plaque deposition and tau hyperphosphorylation

I recently treated a good friend who had a rotator cuff injury similar to mine who suffered the same degree of pain, discomfort, and reduced range of motion