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Description

A novel iron chelator that does not induce HIF-1 activity

coli BL21 (DE3) (Sigma-Aldritch) at 110-fold coverage of the library size, and following 1-hr recovery from transformation, cells were immediately diluted into LB media (Gibco) containing kananycin (selecting for presence of the library) and 10 uM IPTG (inducer for library expression), beginning the massively parallel inhibition measurements