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During all next steps purification steps the fractions were tested by restraint stress and cysteamine methods on experimental animals (Wistar rats)

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To test for irreversibility and the type of inactivation occurring in Prx1 after the hydrogen peroxide pretreatment, 2 OD 600 units of cells were resuspended in 100 l SDSloading buffer containing either DMSO (unmodified), 10 mM TCEP (TCEP reduces disulfides and sulfenic acids only), or 10 mM mmPEG 24 (steady state), and boiled for 5 min at 96C

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