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These are two of the most rigorous and widely adopted methods in peptide manufacturing: SPPS enables precise, stepwise assembly of the amino acid sequence under tightly controlled conditions, while HPLC purification systematically removes synthesis byproducts and sequence variants to yield a high-purity final compound
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To further explore the relationship between glucose-induced GLP-1 release and heat sensitivity, we examined changes in total GLP-1 levels in the serum following glucose administration (Supplementary Fig
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