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Then, 10 l of LacAnc100 or LaccID was added to flat-bottom 96-well plates (Greiner Bio-One, 655101) and 190 l of reaction mixtures containing PBS and different substrates were added with the help of a Multidrop system (Multidrop Combi, Thermo Scientific)
Anionic liposomes have superior solution stability, reduced aggregation in comparison to neutrally charged liposomes, and enhanced endocytosis compared to neutral and cationic liposomes [22, 23]
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