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Importantly for the translational impact of potential EEC-targeted therapies, comparisons between murine and human EECs via RNA-seq have established that receptors expressed in murine models are largely equivalent to those seen in humans 89
There are a multitude of successful differentiation protocols, mostly based on a combination of cell culture medium with different growth supplements, nicotinamide and beta-mercaptoethanol with an average protocol duration of 17 days (Table 3)
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