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Bomfim TR, Forny-Germano L, Sathler LB, et al
Protein precipitation especially prior to flourimetric analysis is the method of choice for extraction in several reports [15, 16] due to its simplicity and greenness as it requires small amount of organic solvent to precipitate the proteins followed by a simple step of centrifugation and filtration as previously discussed under For spiked plasma extraction and analysis in Methods section
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