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Briefly, cells were lysed in the lysis buffer described above, and loaded onto a stepwise sucrose gradient composed of 700-l layers of sucrose of increasing percentage (top-to-bottom: 5%, 10%, 15%, 20%, 25%, and 30%)

Green tea extract and EGCG Green tea extract, specifically its primary catechin EGCG (epigallocatechin gallate), has modest evidence for thermogenesis and fat oxidation