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Since the benefits of hydroxocobalamin over cyanocobalamin have become more apparent, the former has become the main substance used in B12 injections in Europe

In the feet, these nerves carry messages to and from the brain that control sensation, balance, and movement

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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere