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When blood sugar is high, GLP-1 agonists trigger the secretion of insulin, suppress the release of sugar from the liver, and slow the digestion of food
The cell pellet was resuspended and lysed in lysis buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 1% Triton-X100 (v/v), 2 mM MgCl 2 , 0.5 mM TCEP) with protease inhibitors (1 mM PMSF, 1 M pepstatin, 4 M leupeptin, 0.3 M aprotinin) for 30 min at 4 C
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