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13 The retrospective observational design also limited the ability to prove causality between GLP-1RA use and cancer incidence

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Cell viability and proliferation assays The transfected K-562 cells with or without cell sorting and the negative control cells transfected with empty plasmids pcDNA3.1-GST DAF or pcDNA3.1-GST CEAM7 were counted and seeded into 96-well plates in triplicates at a density of 1,000 per well