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Patients not breastfeeding can typically restart GLP-1 therapy 6-8 weeks postpartum once they have been cleared for normal activity by their obstetrician and have stabilized from the immediate postpartum period
Sorted cells were reseeded on Matrigel-pre-coated plates and expanded
Kinase assays for pseudo-substrate characterisation Fifty nanomolar CDK11 p58 -cyclin L (1319)-SAP30BP complex (WT or mutant) was incubated with 6 M GST-SF3B1 1463 and 2 mM ATP in a total volume of 100 L, in assay buffer (150 mM NaCl, 25 mM HEPES-KOH pH 7.9, 9 mM MgCl 2 , 2 mM DTT, 2.5% (v/v) glycerol) for 60 min at RT
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