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After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min

Control animals received equivalent volumes of citrate buffer alone

There have also been a couple of studies of patients with hypothyroidism and diabetes, showing that those patients successfully lost weight, their TSH levels decreased, and their thyroid ended up healthier overall