sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
A glutathione infusion is most often chosen by: physically active people, as support for recovery after hard training or competition, tired and stressed people, when low energy persists despite rest, people who want to support immunity, especially during the infection season, people after a demanding period, for example following an infection or intense mental work
Benefits: L-cysteine from Japan that has been clinically proven to increase glutathione levels in the body
BPC-157 is extensively studied for its role in cellular repair mechanisms, tissue integrity, and protective signaling pathways across muscle, tendon, ligament, and gastrointestinal research models