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At that time, physicians were often reluctant to diagnose this multifaceted clinical syndrome as long COVID because routine diagnostics of blood samples or imaging in survivors of mild acute COVID-19 rarely yielded any pathological results [120]
Visible callus enhancement on imaging typically appears between weeks two and four
After washing with PBS, the stained cells were incubated in serum-free medium and then imaged using fluorescence microscopy (Olympus, Japan) with emission wavelengths of 540 and 575 nm
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