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Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark
Joint pain is not considered one of the most common tirzepatide side effects, but some people report achy joints, stiffness, muscle soreness, or body aches during treatment
Q: Are there any blood markers I should track to confirm my timing protocol is working
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